Anne Le Borgne de Bélisle was born in Port-Royal around 1690 from the union of two families who played a key role in 17th century colonial Acadie. Her parents, Alexandre Le Borgne de Bélisle and Marie de Saint-Étienne de La Tour, ruled Port-Royal in turn. Her paternal and maternal grandfathers, Emmanuel Le Borgne and Charles de Saint-Étienne de La Tour, both merchants, had also both been Governor of Acadie. In 1707, Anne married the king's pilot, Jean-Baptiste Rodrigue, dit de Fonds, who died in 1734. After her husband's death, she continued the family's commercial tradition by heading their fishing enterprise in Louisbourg. With her two sons, Pierre and Michel, she administered the enterprise, which was one of the most prosperous in île Royale (today's Cape Breton), until she married Jean-Baptiste Duperié in 1738.